ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Kangning granules on inflammatory and immunology.</p><p><b>METHOD</b>Pedal swelling induced by 1% agar in mice, dermal capillary hyper-permeability induced by histamine in rats were used to evaluate the anti-inflammatory of Kangning granules, and the clearance rate of intravenous charcoal particles was used to study its effect on non-specific immunologic functions. Serum haemolysin level and the number of antibody secretory cells in spleen were determined to assess its effects of specific immunologic functions. MTT colored method was used to determine the effect of Kangning granules on T- or B-Lymphocytes proliferation.</p><p><b>RESULT</b>5, 10, 20 g x kg(-1) of Kangning Granules significantly inhibited pedal swelling induced by agar in mice (P < 0.05, P < 0.01). 2.4, 4.8, 9.6 g x kg(-1) of Kangning Granules evidently decreased dermal capillary hyper-permeability induced by histamine in rats (P < 0.05, P < 0.01). 10, 20 g x kg(-1) of Kangning Granules enhanced the phagocytic function and phagocytosing velocity in mice (P < 0.05). 5, 10, 20 g x kg(-1) of Kangning Granules obviously increased serum haemolysin level and the number of antibody secretory cells in spleen (P < 0.05, P < 0.01). 20 g x kg(-1) of Kangning Granules promoted B-Lymphocytes proliferation (P < 0.05).</p><p><b>CONCLUSION</b>Kangning granules significantly inhibited inflammatory reaction and increase immune functions in animals.</p>
Subject(s)
Animals , Female , Male , Mice , Rats , Anti-Inflammatory Agents, Non-Steroidal , Pharmacokinetics , Pharmacology , Capillary Permeability , Drug Combinations , Drugs, Chinese Herbal , Pharmacokinetics , Pharmacology , Echinacea , Chemistry , Edema , Pathology , Foot Diseases , Pathology , Immunologic Factors , Pharmacokinetics , Pharmacology , Lymphocyte Activation , Mice, Inbred BALB C , Phagocytosis , Plants, Medicinal , Chemistry , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To observe the variation of ET and CGRP contents in ischemic heart, and NO level in serum of myocardial damaged rats, and their regulation when with the protection of Xinshuping, a traditional Chinese medicine compound.</p><p><b>METHOD</b>The models of myocardial ischemia were prepared by subcutaneous injection of isoproterenol or by ligation of coronary artery.</p><p><b>RESULT</b>ET content in myocardium was significantly increased (P < 0.01), and CGRP content as well as NO level in serum was not changed obviously in the model induced by isoproterenal. However, NO level in serum of rats treated with Xinshuping (ig bid x 2.5 d) was markedly raised (P < 0.01), neither ET not CGRP contents were affected by it. LDH and CK levels in serum of rats were evidently lowered by Xinshuping treatment. S-T segment's elevation of ECG was significantly inhibited and myocardial infarction size was reduced markedly by Xinshuping treatment in rats subjected to coronary artery ligature.</p><p><b>CONCLUSION</b>ET, CGRP or NO is involved in myocardial infarction caused by isoproterenol. The ischemic damage or dysfunction in different models is obviously protected by Xinshuping. The promotion of NO release from vascular endothelium is probably related with this protective effect.</p>